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RT-PCR RNA was extracted from fully matured HBE cells with the AllPrep DNA/RNA Mini kit (80204, QIAGEN AG, Germantown, MD) and treated with DNase I (M0303S, New England BioLabs Inc., Ipswich, MA) before reverse transcription with the iScript cDNA Synthesis Kit (1708891, Bio-Rad)

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In 2022, Rhode Island had the fourth-highest rate of overdose deaths involving cocaine in 2022, after Washington, D.C., Delaware, and Vermont, according to the Centers for Disease Control and Prevention

Cigarette use by pregnant women has also been shown to cause birth defects, including mental and physical disabilities In the early times, people could buy cigarettes and smoke pretty much anywhere even in hospitals

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